Article
Biases in human papillomavirus genotype prevalence assessment associated with commonly used consensus primers.
International journal of cancer - 1 Jan 2006
Chan Paul K S, Cheung Tak-Hong, Tam Ann O Y, Lo Keith W K, Yim So-Fan, Yu May M Y, To Ka-Fai, Wong Yick-Fu, Cheung Jo L K, Chan Denise P C, Hui Mamie, Ip Margaret
Abstract excerpt
Consensus primers targeting human papillomaviruses (HPVs) have biases in sensitivity toward certain HPV types. We applied 3 primer sets (GP5+/6+, MY09/11, PGMY09/11) in parallel on 120 Chinese cervical cancer specimens. GP5+/6+ exhibited a poor sensitivity for HPV52, for which the prevalence among squamous cell cervical cancer was underestimated from 14.6% to 0%. The fact that HPV52 should rank second in...
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