Article
Cloning and whole nucleotide sequence of the gene for the light chain component of botulinum type E toxin from Clostridium butyricum strain BL6340 and Clostridium botulinum type E strain Mashike.
Microbiology and immunology - 1 Jan 1992
Fujii N, Kimura K, Yashiki T, Tsuzuki K, Moriishi K, Yokosawa N, Syuto B, Oguma K
Abstract excerpt
Chromosomal DNAs were extracted from Clostridium butyricum strain BL6340 and Clostridium botulinum type E strain Mashike. The 6.0 Kbp fragment coding for the entire light chain (L) component and the N-terminus of heavy chain (H) component of botulinum type E toxin was obtained from each extracted DNAs after digestion with HindIII. The entire nucleotide sequences for the light chain components of these cloned...
Topics
- Amino Acid Sequence
- Antibodies, Monoclonal
- Base Sequence
- Blotting, Western
- Botulinum Toxins
- Cloning, Molecular
- Clostridium
- Clostridium botulinum
- DNA, Bacterial
- Genes, Bacterial