Article
[Establishment of the genotype-specific targets of botulinum neurotoxins types B and E based on reverse genetics].
Nan fang yi ke da xue xue bao = Journal of Southern Medical University - 1 Aug 2008
Li Ying, Yang Chun-li, Zhao Ying, Guo Yu-qi, Liu Chang-zheng, Zhou Xiao-hong
Abstract excerpt
OBJECTIVE: To establish the genotype-specific targets plasmids and engineered E.coli strains of botulinum neurotoxins (BoNT) types B and E based on reverse genetics. METHODS: The gene sequences of BoNT were obtained from GenBank and analyzed using DNAMAN, Lasergene, Vector NTI and BLAST. Two target fragments of BoNT/B and BoNT/E were anchored and then synthesized as 5 and 10 short DNA single strands,...
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