Article
Enhancement of glutamine utilization in Bacillus subtilis through the GlnK-GlnL two-component regulatory system.
Journal of bacteriology - 1 Jul 2005
Satomura Takenori, Shimura Daisuke, Asai Kei, Sadaie Yoshito, Hirooka Kazutake, Fujita Yasutaro
Abstract excerpt
During DNA microarray analysis, we discovered that the GlnK-GlnL (formerly YcbA-YcbB) two-component system positively regulates the expression of the glsA-glnT (formerly ybgJ-ybgH) operon in response to glutamine in the culture medium on Northern analysis. As a result of gel retardation and DNase I footprinting analyses, we found that the GlnL protein interacts with a region (bases -13 to -56; +1 is the...
Topics
- Bacillus subtilis
- Bacterial Proteins
- Base Sequence
- Carrier Proteins
- DNA Primers
- Genes, Reporter
- Genotype
- Glutaminase
- Glutamine
- Histidine Kinase
- Kinetics
- Molecular Sequence Data
- Protein Kinases
- Trans-Activators