Article
Three of four GlnR binding sites are essential for GlnR-mediated activation of transcription of the Amycolatopsis mediterranei nas operon.
Journal of bacteriology - 1 Jun 2013
Wang Ying, Wang Jing-Zhi, Shao Zhi-Hui, Yuan Hua, Lu Yin-Hua, Jiang Wei-Hong, Zhao Guo-Ping, Wang Jin
Abstract excerpt
In Amycolatopsis mediterranei U32, genes responsible for nitrate assimilation formed one operon, nasACKBDEF, whose transcription is induced by the addition of nitrate. Here, we characterized GlnR as a direct transcriptional activator for the nas operon. The GlnR-protected DNA sequences in the promoter region of the nas operon were characterized by DNase I footprinting assay, the previously deduced Streptomyces...
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