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Article

A rapid polymerase chain reaction method for identifying fixed specimens.

Electrophoresis - 1 Mar 1992

Gill P, Kimpton C P, Sullivan K

Abstract excerpt

DNA profiling analysis of preserved tissue samples was achieved by using an extraction method which incorporated the use of a chelating resin "Chelex" to chelate inhibitory polyvalent metal ions. An automated sequencer was used to estimate sizes of low molecular weight trimeric and tetrameric microsatellites labelled with fluorescent dyes. The entire analysis can be completed within 48h. Probabilities of chance...

Topics

Alleles
Base Sequence
Chelating Agents
DNA
DNA, Neoplasm
DNA, Satellite
Evaluation Studies as Topic
Humans
Ion Exchange Resins
Male
Molecular Sequence Data
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Polystyrenes

Polyvinyls

Prostatic Neoplasms

Tissue Preservation

Source

Identifiers

PMID: 1592049
DOI: 10.1002/elps.1150130135

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1592049-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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