Use of principal components analysis for mutation detection with two-dimensional electrophoresis protein separations.

Abstract excerpt

The application of two-dimensional electrophoresis (2-DE) to mutation detection requires the capability to monitor each protein in a 2-DE pattern for significant changes in abundance indicative of a mutation event. Previously, mutation searches were done using a univariate outlier detection method in which each protein spot was considered independently in a classical outlier search. An alternative approach to...

Topics

• Animals
• Electrophoresis, Gel, Two-Dimensional
• Evaluation Studies as Topic
• Female
• Male
• Mice
• Multivariate Analysis
• Mutation
• Proteins