Article
Ferredoxin-NADP+ reductase. Kinetics of electron transfer, transient intermediates, and catalytic activities studied by flash-absorption spectroscopy with isolated photosystem I and ferredoxin.
The Journal of biological chemistry - 15 Jul 2005
Cassan Nicolas, Lagoutte Bernard, Sétif Pierre
Abstract excerpt
The electron transfer cascade from photosystem I to NADP+ was studied at physiological pH by flash-absorption spectroscopy in a Synechocystis PCC6803 reconstituted system comprised of purified photosystem I, ferredoxin, and ferredoxin-NADP+ reductase. Experiments were conducted with a 34-kDa ferredoxin-NADP+ reductase homologous to the chloroplast enzyme and a 38-kDa N-terminal extended form. Small differences in...
Topics
- Catalysis
- Dose-Response Relationship, Drug
- Electrons
- Ferredoxin-NADP Reductase
- Ferredoxins
- Gene Deletion
- Kinetics
- Models, Chemical
- Mutation
- NADP
- Oxygen
- Photosystem I Protein Complex
- Protein Binding
- Spectrophotometry
- Synechocystis
- Time Factors