Article
Measurement of photosystem I activity with photoreduction of recombinant flavodoxin.
Analytical biochemistry - 15 Nov 1998
Zhao J, Li R, Bryant D A
Abstract excerpt
Flavodoxin can function as an alternative electron acceptor for photosystem I (PSI) in place of ferredoxin under iron-limiting conditions. The isiB gene, encoding the flavodoxin in Synechococcus sp. PCC 7002, was overexpressed in Escherichia coli. Under the conditions employed, most recombinant f...
Topics
- Bacterial Proteins
- Cross-Linking Reagents
- Cyanobacteria
- Escherichia coli
- Flavodoxin
- Hydrogen-Ion Concentration
- Intracellular Membranes
- Isopropyl Thiogalactoside
- Kinetics
- Magnesium
- Mutation
- Oxidation-Reduction
- Photosynthesis
- Photosynthetic Reaction Center Complex Proteins
- Photosystem I Protein Complex
- Recombinant Proteins
- Spectrum Analysis
- Urea