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A strategy for the generation of specific human antibodies by directed evolution and phage display

1 May 2005

Abstract excerpt

This study describes the construction of a library of single-chain antibody fragments (scFvs) from a single human donor by individual amplification of all heavy and light variable domains (1.1 x 10(8) recombinants). The library was panned using the phage display technique, which allowed selection of specific scFvs (3F and C1) capable of recognizing Cn2, the major toxic component of Centruroides noxius scorpion...

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Share to Monoclonal and Polyclonal Antibodies Research
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Identifiers

PMID: 15885107
DOI: 10.1111/j.1742-4658.2005.04687.x

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