Article
Detecting differential expression of parental or progenitor alleles in genetic hybrids and allopolyploids.
Methods in enzymology - 1 Jan 2005
Pikaard Craig S, Preuss Sasha, Earley Keith, Lawrence Richard J, Lewis Michelle S, Chen Z Jeffrey
Abstract excerpt
Three assays useful for detecting specific RNA transcripts are primer extension, S1 nuclease protection, and reverse-transcription-cleaved amplified polymorphic sequence (RT-CAPS) analysis. All three of these techniques are used routinely for gene expression analyses and allow insights not possible by RNA blot (northern blot) hybridization. In this chapter, we describe how the primer extension, S1 nuclease...
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