Article
High-throughput RNA-seq for allelic or locus-specific expression analysis in Arabidopsis-related species, hybrids, and allotetraploids.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2014
Ng Danny W-K, Shi Xiaoli, Nah Gyoungju, Chen Z Jeffrey
Abstract excerpt
With the next generation sequencing technology, RNA-Seq (RNA sequencing) becomes one of the most powerful tools in quantification of global transcriptomes, discovery of new transcripts and alternative isoforms, as well as detection of single nucleotide polymorphisms (SNPs). RNA-Seq is advantageous over hybridization-based gene quantification methods: (1) it does not require prior information about genomic...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.