Article
Microarray-based genotyping for blood groups: comparison of gene array and 5'-nuclease assay techniques with human platelet antigen as a model.
Transfusion - 1 May 2005
Bugert Peter, McBride Simon, Smith Graham, Dugrillon Alex, Klüter Harald, Ouwehand Willem H, Metcalfe Paul
Abstract excerpt
BACKGROUND: Most blood group alloantigens specific for red cells and platelets (PLTs) are based on single-nucleotide polymorphisms (SNPs) in genes encoding relevant membrane proteins. STUDY DESIGN AND METHODS: By use of five human PLT antigen (HPA) systems as a model, the suitability of a fourth-generation microarray technique for SNP typing was investigated. The results of the former were compared with those of...
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