Article
Immobilization of the glycosylphosphatidylinositol-anchored Gas1 protein into the chitin ring and septum is required for proper morphogenesis in yeast.
Molecular biology of the cell - 1 Nov 2009
Rolli Eleonora, Ragni Enrico, Calderon Julia, Porello Silvia, Fascio Umberto, Popolo Laura
Abstract excerpt
Gas1p is a glucan-elongase that plays a crucial role in yeast morphogenesis. It is predominantly anchored to the plasma membrane through a glycosylphosphatidylinositol, but a fraction was also found covalently bound to the cell wall. We have used fusions with the green fluorescent protein or red fluorescent protein (RFP) to determine its localization. Gas1p was present in microdomains of the plasma membrane, at...
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