Article
The pyruvate requirement of some members of the Mycobacterium tuberculosis complex is due to an inactive pyruvate kinase: implications for in vivo growth.
Molecular microbiology - 1 Apr 2005
Keating Lisa A, Wheeler Paul R, Mansoor Huma, Inwald Jacqueline K, Dale James, Hewinson R Glyn, Gordon Stephen V
Abstract excerpt
Through examination of one of the fundamental in vitro characteristics of Mycobacterium bovis--its requirement for pyruvate in glycerol medium--we have revealed a lesion in central metabolism that has profound implications for in vivo growth and nutrition. Not only is M. bovis unable to use glycerol as a sole carbon source but the lack of a functioning pyruvate kinase (PK) means that carbohydrates cannot be used...
Topics
- Animals
- Base Sequence
- Carbon
- Culture Media
- Glycerol
- Glycerol Kinase
- Humans
- Molecular Sequence Data
- Mutation
- Mycobacterium bovis
- Mycobacterium tuberculosis