Article
In vitro mutation and selection of doubled-haploid Brassica napus lines with improved resistance to Sclerotinia sclerotiorum.
Plant cell reports - 1 Jun 2005
Liu S, Wang H, Zhang J, Fitt B D L, Xu Z, Evans N, Liu Y, Yang W, Guo X
Abstract excerpt
This paper describes a new protocol to develop doubled-haploid (DH) Brassica napus lines with improved resistance to Sclerotinia sclerotiorum. In this protocol, haploid seedlings derived from microspore cultures of B. napus were used to produce haploid calli for in vitro mutation-selection. For routine screening, mutation was induced by EMS (ethylmethane sulfonate) or occurred spontaneously, and screening for...
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