Article
Forward and reverse genetics of rapid-cycling Brassica oleracea.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Mar 2009
Himelblau Edward, Gilchrist Erin J, Buono Kelly, Bizzell Colleen, Mentzer Laurie, Vogelzang Robert, Osborn Thomas, Amasino Richard M, Parkin Isobel A P, Haughn George W
Abstract excerpt
Seeds of rapid-cycling Brassica oleracea were mutagenized with the chemical mutagen, ethylmethane sulfonate. The reverse genetics technique, TILLING, was used on a sample population of 1,000 plants, to determine the mutation profile. The spectrum and frequency of mutations induced by ethylmethane sulfonate was similar to that seen in other diploid species such as Arabidopsis thaliana. These data indicate that the...
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