Article
Synthesis of reassortant infectious bursal disease virus in chickens injected directly with infectious clones from different virus strains.
Acta biochimica et biophysica Sinica - 1 Mar 2005
Li Long, Huang Yao-Wei, Wang Lian-Sheng, Wan Wang-Jun, Yu Lian
Abstract excerpt
The infectious bursal disease virus (IBDV), a member of the Birnaviridae family, containing a bisegmented double-stranded RNA genome, encodes four structural viral proteins, VP1, VP2, VP3, and VP4, as well as a non-structural protein, VP5. In the present paper, the segment A from two IBDV strains, field isolate ZJ2000 and attenuated strain HZ2, were inserted into one NaeI site by site-directed silent mutagenesis...
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