Article
The phenotype of in vitro reconstituted normal human oral epithelium is essentially determined by culture medium.
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology - 1 Apr 2005
Costea Daniela Elena, Dimba Anne Okumo Elizabeth, Loro Lado Lako, Vintermyr Olav Karsten, Johannessen Anne Christine
Abstract excerpt
OBJECTIVE: To evaluate the role of various culture media and serum supplement on growth of oral cells in monolayer, and on morphogenesis of in vitro reconstituted normal human oral epithelium. METHODS: Primary keratinocytes and fibroblasts were isolated from normal human buccal mucosa. The monolayers were assessed by growth curve analysis and morphology. The organotypic cultures were evaluated by morphometry,...
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