Article
Construction of large area organotypical cultures of oral mucosa and skin.
Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology - 1 Aug 2003
Igarashi M, Irwin C R, Locke M, Mackenzie I C
Abstract excerpt
BACKGROUND: Skin and oral mucosal keratinocytes grown in vitro usually lose their normal patterns of differentiation, unless grown as organotypical cultures that are constructed by allowing collagen gels containing fibroblasts to contract before they are plated with keratinocytes and raised to the air/medium interface. However, the contraction process tends to produce small irregular cultures. METHODS: To...
Topics
- Adult
- Animals
- Cell Differentiation
- Cell Division
- Collagen
- Culture Media
- Culture Techniques
- Dogs
- Epithelial Cells
- Fibroblasts
- Gels