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Article

Bcl-XL as a fusion protein for the high-level expression of membrane-associated proteins.

Protein science : a publication of the Protein Society - 1 Apr 2005

Thai Khang, Choi Jungyuen, Franzin Carla M, Marassi Francesca M

Abstract excerpt

An Escherichia coli plasmid vector for the high-level expression of hydrophobic membrane proteins is described. The plasmid, pBCL, directs the expression of a target polypeptide fused to the C terminus of a mutant form of the anti-apoptotic Bcl-2 family protein, Bcl-XL, where the hydrophobic C terminus has been deleted, and Met residues have been mutated to Leu to facilitate CNBr cleavage after a single Met...

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Identifiers

PMID: 15741345
DOI: 10.1110/ps.041244305
PMCID: PMC2253446

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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