Article
Mutational analysis of the stator subunit E of the yeast V-ATPase.
The Journal of biological chemistry - 6 May 2005
Owegi Margaret A, Carenbauer Anne L, Wick Nicole M, Brown Jamie F, Terhune Kari L, Bilbo Sarah A, Weaver Rebecca S, Shircliff Rebecca, Newcomb Natalia, Parra-Belky Karlett J
Abstract excerpt
Subunit E is a component of the peripheral stalk(s) that couples membrane and peripheral subunits of the V-ATPase complex. In order to elucidate the function of subunit E, site-directed mutations were performed at the amino terminus and carboxyl terminus. Except for S78A and D233A/T202A, which exhibited V(1)V(o) assembly defects, the function of subunit E was resistant to mutations. Most mutations complemented...
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