Stairway assays: rapid localization of multiple protein/DNA interaction sites in gene-regulatory 5' regions.

Article

Stairway assays: rapid localization of multiple protein/DNA interaction sites in gene-regulatory 5' regions.

BioTechniques - 1 Mar 1992

van Wijnen A J, Bidwell J, Lian J B, Stein J L, Stein G S

Abstract excerpt

We describe a rapid method for initial localization of protein/DNA-binding sites in large DNA segments, even when biological information is limited. The procedure combines the gel retardation assay with bidirectional deletion analysis using restriction enzymes. Electrophoresis of binding reaction...

Topics

Animals
Binding Sites
Biotechnology
Chromosome Deletion
DNA
DNA-Binding Proteins
Genes, Regulator
Genetic Techniques
Humans
Mutation
Promoter Regions, Genetic

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