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Article

Stairway assays: rapid localization of multiple protein/DNA interaction sites in gene-regulatory 5' regions.

BioTechniques - 1 Mar 1992

van Wijnen A J, Bidwell J, Lian J B, Stein J L, Stein G S

Abstract excerpt

We describe a rapid method for initial localization of protein/DNA-binding sites in large DNA segments, even when biological information is limited. The procedure combines the gel retardation assay with bidirectional deletion analysis using restriction enzymes. Electrophoresis of binding reactions with a bidirectional set of progressively shortened probes results in a stairway pattern of both uncomplexed DNA and...

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PMID: 1571151

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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