Article
Application of fluorescence-based resistance gene analog analysis for genotyping plant genetic resources.
Biotechnology letters - 1 Jan 2005
Cheng Jianping, Yan Jun, Krugman Tamar, Fahima Tzion
Abstract excerpt
Conventional approaches for detecting disease resistance gene analogs (RGAs) in plants are based on agarose gels or on polyacrylamide gel electrophoresis (PAGE) in combination with silver staining or radioactive labeling. A modified method for RGA analysis has been developed by using fluorescence-labeled primers for PCR amplifications. The amplified fragments are detected by denaturing PAGE using an automated...
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