Article
Retrotransposon-based insertion polymorphisms (RBIP) for high throughput marker analysis.
The Plant journal : for cell and molecular biology - 1 Dec 1998
Flavell A J, Knox M R, Pearce S R, Ellis T H
Abstract excerpt
Two assays based upon PCR detection of a polymorphic PDR1 retrotransposon insertion in Pisum sativum have been developed. Both methods involve PCR with primers derived from the transposon and flanking DNA. The first method uses a dot assay for PCR product detection which could be fully automated for handling thousands of samples. The second method, which is designed to handle lower numbers, requires a single PCR...
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