Article
Use of Molecular Markers for Doubled Haploid Technology: From Academia to Plant Breeding Companies.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Tuvesson Stine Due, Larsson Clas-Tomas, Ordon Frank
Abstract excerpt
Molecular markers are employed for doubled haploid (DH) technology by researchers and applied plant breeders in many crops. In the 1990s, isozymes and RFLPs were commonly used marker technologies to characterize DHs and were later replaced by PCR- based markers (e.g., RAPDs, AFLPs, ISSRs, SSRs) and today by SNPs. Markers are used for multiple purposes in DH production, that is, for the study of genes underlying...
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