Article
Insertional mutagenesis in a homologue of a Pi transporter gene confers arsenate resistance on chlamydomonas.
Plant & cell physiology - 1 Jun 2003
Kobayashi Isao, Fujiwara Shoko, Shimogawara Kosuke, Kaise Toshikazu, Usuda Hideaki, Tsuzuki Mikio
Abstract excerpt
An arsenate-resistant mutant AR3 of Chlamydomonas reinhardtii is a recessive mutant generated by random insertional mutagenesis using the ARG7 gene. AR3 shows about 10-fold resistance against arsenate toxicity compared with the wild type. By using a flanking region of an inserted tag as a probe, we cloned the corresponding wild-type allele (PTB1) of a mutated gene, which could completely complement the...
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