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Article

Characterization of Mos1-mediated mutagenesis in Caenorhabditis elegans: a method for the rapid identification of mutated genes.

Genetics - 1 Mar 2005

Williams Daniel C, Boulin Thomas, Ruaud Anne-Françoise, Jorgensen Erik M, Bessereau Jean-Louis

Abstract excerpt

Insertional mutagenesis with a heterologous transposon provides a method to rapidly determine the molecular identity of mutated genes. The Drosophila transposon Mos1 can be mobilized to cause mutations in Caenorhabditis elegans (Bessereau et al. 2001); however, the mutagenic rate was initially too low for use in most forward genetic screens. To increase the effectiveness of Mos1-mediated mutagenesis we examined...

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Identifiers

PMID: 15654093
DOI: 10.1534/genetics.104.038265
PMCID: PMC1449563

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