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Article

Development and validation of PCR primers to assess the diversity of Clostridium spp. in cheese by temporal temperature gradient gel electrophoresis.

Applied and environmental microbiology - 1 Jan 2005

Le Bourhis Anne-Gaëlle, Saunier Katiana, Doré Joël, Carlier Jean-Philippe, Chamba Jean-François, Popoff Michel-Robert, Tholozan Jean-Luc

Abstract excerpt

A nested-PCR temporal temperature gradient gel electrophoresis (TTGE) approach was developed for the detection of bacteria belonging to phylogenetic cluster I of the genus Clostridium (the largest clostridial group, which represents 25% of the currently cultured clostridial species) in cheese suspected of late blowing. Primers were designed based on the 16S rRNA gene sequence, and the specificity was confirmed in...

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Identifiers

PMID: 15640166
DOI: 10.1128/AEM.71.1.29-38.2005
PMCID: PMC544257

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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