Article
RNA polymerase III catalysed transcription can be regulated in Saccharomyces cerevisiae by the bacterial tetracycline repressor-operator system.
The EMBO journal - 1 Apr 1992
Dingermann T, Frank-Stoll U, Werner H, Wissmann A, Hillen W, Jacquet M, Marschalek R
Abstract excerpt
We have investigated whether the RNA polymerase III-driven transcription of eukaryotic tRNA genes can be regulated by the prokaryotic tetracycline operator-repressor system. The bacterial tet operator (tetO) was inserted at two different positions (-7 and -46) upstream of a tRNA(Glu) (amber) suppressor gene. Both constructs are transcribed in Saccharomyces cerevisiae and yield functional tRNAs as scored by...
Topics
- Alleles
- Animals
- Base Sequence
- Cloning, Molecular
- DNA-Binding Proteins
- Dictyostelium
- Gene Expression Regulation, Fungal
- Genes, Bacterial
- Genes, Suppressor
- Molecular Sequence Data