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Monoclonal antibodies expression improvement in CHO cells by PiggyBac transposition regarding vectors ratios and design

29 Jun 2017

Abstract excerpt

Establishing stable Chinese Hamster Ovary (CHO) cells producing monoclonal antibodies (mAbs) usually pass through the random integration of vectors to the cell genome, which is sensitive to gene silencing. One approach to overcome this issue is to target a highly transcribed region in the genome. Transposons are useful devices to target active parts of genomes, and PiggyBac (PB) transposon can be considered as a...

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PMID: 28662065
DOI: 10.1371/journal.pone.0179902

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