Article
Deletion of the dynein heavy-chain gene DYN1 leads to aberrant nuclear positioning and defective hyphal development in Candida albicans.
Eukaryotic cell - 1 Dec 2004
Martin R, Walther A, Wendland J
Abstract excerpt
Cytoplasmic dynein is a microtubule-associated minus-end-directed motor protein. CaDYN1 encodes the single dynein heavy-chain gene of Candida albicans. The open reading frames of both alleles of CaDYN1 were completely deleted via a PCR-based approach. Cadyn1 mutants are viable but grow more slowly than the wild type. In vivo time-lapse microscopy was used to compare growth of wild-type (SC5314) and dyn1 mutant...
Topics
- Active Transport, Cell Nucleus
- Alleles
- Candida albicans
- Cell Movement
- Cell Nucleus
- Cell Survival
- Cytoplasm
- DNA Primers
- Dyneins
- Gene Deletion
- Genotype
- Green Fluorescent Proteins
- Heterozygote
- Microscopy, Fluorescence