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Article

Synaptogenesis and amino acid release from long term embryonic rat spinal cord neuronal culture using tissue culture inserts.

Journal of neuroscience methods - 30 Jan 2005

Marsala Martin, Kakinohana Osamu, Hefferan Michael P, Cizkova Dasa, Kinjoh Kiyohiko, Marsala Silvia

Abstract excerpt

In the present study, using tissue culture inserts (TCI) coupled with a primary spinal cord neuronal culture, we characterize a new perfusion system, which permits continuous perfusate collection from cultured neurons. Primary spinal cord neurons were isolated from the lumbar portion of E14 spinal cords of Sprague-Dawley rats, plated on TCI and fed with DMEM/B27/10% FBS. At 1-4 weeks after isolation the...

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Identifiers

PMID: 15585285
DOI: 10.1016/j.jneumeth.2004.05.011

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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