Article
Genotyping SNPs with the LightCycler.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2005
Lareu María Victoria, Ruiz-Ponte Clara
Abstract excerpt
Here, a single nucleotide polymorphism typing methodology is described based on polymerase chain reaction monitoring, in real time, of fluorescently labeled amplified products using the LightCycler. The main advantages of the system are the time required for the analysis (about 20 min), combined with the robustness, accuracy, and the sensitivity of the method.
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