Article
PCR-restriction fragment length polymorphism assay for detection of gyrA mutations associated with fluoroquinolone resistance in Campylobacter coli.
Antimicrobial agents and chemotherapy - 1 Dec 2004
Alonso Rodrigo, Mateo Estibaliz, Girbau Cecilia, Churruca Estibaliz, Martinez Irati, Fernández-Astorga Aurora
Abstract excerpt
A fragment of the gyrA gene was sequenced from 34 isolates of Campylobacter coli, including 23 isolates resistant to ciprofloxacin. All ciprofloxacin-resistant isolates examined by DNA sequencing carried a point mutation at position Thr-86 on the gyrA gene product, involving the replacement of Thr-86 by Ile. A combined PCR-restriction fragment length polymorphism technique using RsaI was developed to detect this...
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