Article
Protease domain of human ADAM33 produced by Drosophila S2 cells.
Protein expression and purification - 1 Dec 2004
Prosise Winifred W, Yarosh-Tomaine Taisa, Lozewski Zia, Ingram Richard N, Zou Jun, Liu Jian-Jun, Zhu Feng, Taremi S Shane, Le Hung V, Wang Wenyan
Abstract excerpt
Human ADAM33 is a multiple-domain, type-I transmembrane zinc metalloprotease recently implicated in asthma susceptibility [Nature 418 (2002) 426]. To provide an active protease for functional studies, expression of a recombinant ADAM33 zymogen (pro-catalytic domains, pro-CAT) was attempted in several insect cells. The pro-CAT was cloned into baculovirus under the regulation of the polyhedron promoter and using...
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