Cloning, analysis, and heterologous expression of a polyketide synthase gene cluster of Streptomyces curacoi.

doi:10.1111/j.1470-8744.1992.tb00197.x

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Article

Cloning, analysis, and heterologous expression of a polyketide synthase gene cluster of Streptomyces curacoi.

Biotechnology and applied biochemistry - 1 Feb 1992

Bergh S, Uhlén M

Abstract excerpt

Streptomyces curacoi produces curamycin, an antibiotic based on a modified orsellinic acid skeleton that is synthesized by the polyketide pathway. We have cloned, characterized, and partly sequenced a polyketide synthase gene cluster of S. curacoi. The sequence data reveal an organization of open...

Topics

Amino Acid Sequence
Base Sequence
Blotting, Southern
Cloning, Molecular
DNA, Bacterial
Gene Expression
Genes, Bacterial
Molecular Sequence Data
Multienzyme Complexes
Multigene Family
Phenotype
Restriction Mapping
Streptomyces

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