Article
Purification, characterisation and intracellular localisation of aryl hydrocarbon interacting protein-like 1 (AIPL1) and effects of mutations associated with inherited retinal dystrophies.
Biochimica et biophysica acta - 14 Oct 2004
Gallon Victoria A, Wilkie Susan E, Deery Evelyne C, Newbold Richard J, Sohocki Melanie M, Bhattacharya Shomi S, Hunt David M, Warren Martin J
Abstract excerpt
Mutations in AIPL1 are associated with Leber Congenital Amaurosis (LCA), a major cause of childhood blindness, yet the cellular function of the encoded protein has yet to be fully elucidated. In order to investigate the biochemistry of AIPL1, we have developed a system for the expression of the recombinant protein in bacteria and its subsequent purification. The secondary structure and thermostability of...
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