Article
Cloning, expression and characterization of a sialidase gene from Arthrobacter ureafaciens.
Biotechnology and applied biochemistry - 1 Jun 2005
Christensen Søren, Egebjerg Jan
Abstract excerpt
Sialidases have recently been used in the processing of clinically relevant asialoproteins. The Arthrobacter ureafaciens sialidase (EC 3.2.1.18) exhibits broad substrate specificity and is often used in such applications. We have employed an expression cloning strategy to isolate the A. ureafaciens sialidase. The clone encodes a 990-amino-acid 104 kDa open-reading-frame protein containing three domains: an...
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