Article
A reverse genetics study of resistance to neuraminidase inhibitors in an influenza A/H1N1 virus.
Antiviral therapy - 1 Aug 2004
Abed Yacine, Goyette Nathalie, Boivin Guy
Abstract excerpt
A system of reverse genetics was used to generate influenza A/H1N1 viruses harbouring neuraminidase (NA) mutations previously associated with resistance to NA inhibitors in various viral subtypes. The His274Tyr and Glu119Gln mutants were rescued whereas the Arg292Lys and Glu1l9 --> Gly, Val, Ala or Asp mutants could not be generated. In NA inhibition assays, the His274Tyr mutant was resistant to oseltamivir...
Topics
- Acetamides
- Acids, Carbocyclic
- Antiviral Agents
- Cyclopentanes
- Drug Resistance, Viral
- Enzyme Inhibitors
- Guanidines
- Humans
- Influenza A Virus, H1N1 Subtype
- Influenza A virus
- Inhibitory Concentration 50
- Mutation
- Neuraminidase
- Oseltamivir
- Pyrans
- Recombination, Genetic
- Sialic Acids
- Zanamivir