Article
Proximal cysteine residue is essential for the enzymatic activities of cytochrome P450cam.
European journal of biochemistry - 1 Jan 2001
Yoshioka S, Takahashi S, Hori H, Ishimori K, Morishima I
Abstract excerpt
To investigate the functional and structural roles of the proximal thiolate ligand in cytochrome P450cam, we prepared the C357H mutant of the enzyme in which the axial cysteine residue (Cys357) was replaced with a histidine residue. We obtained the unstable C357H mutant by developing a new preparation procedure involving in vitro folding of P450cam from the inclusion bodies. The C357H mutant in the ferrous-CO...
Topics
- Benzene Derivatives
- Camphor
- Camphor 5-Monooxygenase
- Carbon Monoxide
- Catalytic Domain
- Cysteine
- Ferric Compounds
- Ferrous Compounds
- Histidine
- Ligands
- Mutation