Article
Detection of cariogenic bacteria genes by a combination of allele-specific polymerase chain reactions and a novel bioluminescent pyrophosphate assay.
Analytical biochemistry - 15 Oct 2004
Arakawa Hidetoshi, Karasawa Koji, Igarashi Takeshi, Suzuki Shigeya, Goto Nobuichi, Maeda Masako
Abstract excerpt
We developed a novel bioluminescent assay for detection of pyrophosphate in polymerase chain reaction (PCR) product. The principle of this method is as follows: pyrophosphate released by PCR is converted to adenosine 5'-triphosphate (ATP) by pyruvate phosphate dikinase in the presence of the substrate pyruvate phosphate and the coenzyme adenosine 5'-monophosphate; subsequently, ATP concentration is determined by...
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