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Real-time PCR for quantification ofStreptococcus mutans

1 Nov 2002

Abstract excerpt

A real-time polymerase chain reaction (PCR) assay was developed for the quantification of Streptococcus mutans. Primers targeting gtf genes of S. mutans were designed and tested for their specificity using 28 oral streptococcal strains, three other bacterial strains, and human DNA. The primers could amplify specifically the target DNA fragment from a mixture of oral streptococcus genomic DNA containing about 10...

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PMID: 12445641
DOI: 10.1111/j.1574-6968.2002.tb11451.x

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