Article
Detection of cariogenic bacterial genes by microchip electrophoresis.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences - 15 Oct 2004
Karasawa Koji, Arakawa Hidetoshi, Igarashi Takeshi, Goto Nobuichi, Maeda Masako
Abstract excerpt
Allele-specific PCR primers were designed, based on the dextranase (dex) gene, to identify Streptococcus mutans and Streptococcus sobrinus in dental plaque; subsequently, PCR products were detected via microchip electrophoresis (ME). In order to amplify the dex gene fragment of S. mutans and S. sobrinus, the following two PCR methods were established. Duplex allele-specific PCR primers were designed on a region...
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