Article
The HLA-A,B "negative" mutant cell line C1R expresses a novel HLA-B35 allele, which also has a point mutation in the translation initiation codon.
Journal of immunology (Baltimore, Md. : 1950) - 15 Mar 1992
Zemmour J, Little A M, Schendel D J, Parham P
Abstract excerpt
The HLA-A,B negative mutant cell line C1R is widely used as a transfection recipient in functional studies of class I MHC genes. It was derived from a normal B cell line, Hmy2, by three rounds of mutagenesis and immunoselection with anti-HLA mAb. Serology characterizes C1R to be negative for the HLA-A2, A3, B35, Bw62, and Cw3 Ag of the parental cell line while retaining expression of HLA-Cw4. We find, however,...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Cell Line
- Gene Expression
- Genes
- HLA-A2 Antigen
- HLA-B35 Antigen
- Humans
- Molecular Sequence Data