Article
Fusidic acid-resistant mutants of Salmonella enterica serovar typhimurium have low levels of heme and a reduced rate of respiration and are sensitive to oxidative stress.
Antimicrobial agents and chemotherapy - 1 Oct 2004
Macvanin Mirjana, Ballagi Andras, Hughes Diarmaid
Abstract excerpt
Mutations in the translation elongation factor G (EF-G) make Salmonella enterica serovar Typhimurium resistant to the antibiotic fusidic acid. Fus(r) mutants are hypersensitive to oxidative stress and rapidly lose viability in the presence of hydrogen peroxide. We show that this phenotype is associated with reduced activity of two catalase enzymes, HPI (a bifunctional catalase-hydroperoxidase) and HPII (a...
Topics
- Anti-Bacterial Agents
- Catalase
- Culture Media
- DNA Damage
- Drug Resistance, Bacterial
- Fusidic Acid
- Genes, Bacterial
- Genotype
- Heme
- Hydrogen Peroxide
- Iron
- Kinetics
- Lac Operon
- Mutation
- Oxidative Stress