Article
High-sensitivity detection of the A3243G mutation of mitochondrial DNA by a combination of allele-specific PCR and peptide nucleic acid-directed PCR clamping.
Clinical chemistry - 1 Nov 2004
Urata Michiyo, Wada Yui, Kim Sang Ho, Chumpia Worawan, Kayamori Yuzo, Hamasaki Naotaka, Kang Dongchon
Abstract excerpt
BACKGROUND: The A3243G mutation of mitochondrial DNA (mtDNA) is involved in many common diseases, including diabetes mellitus and mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS). For detection of this mutation, allele-specific PCR is highly sensitive but requires strict control of PCR conditions; it thus is not adequate for a routine clinical test. We aimed to develop a...
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