Article
Detection of food-borne pathogens with DNA arrays on disk.
Talanta - 15 Nov 2012
Arnandis-Chover T, Morais S, Tortajada-Genaro L A, Puchades R, Maquieira Á, Berganza J, Olabarria G
Abstract excerpt
A DNA oligonucleotide array for duplex pathogen detection on a DVD platform is developed. The assay involves hybridization of PCR products and optical detection using compact disc technology. Different DNA array constructions for attachment of synthetic oligonucleotides on to DVD surface are evaluated, finding that streptavidin-biotin coupling method yielded the highest sensitivity in combination with enzymatic...
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