Article
Elements in the N-terminal signaling sequence that determine cytosolic topology of short-chain dehydrogenases/reductases. Studies with retinol dehydrogenase type 1 and cis-retinol/androgen dehydrogenase type 1.
The Journal of biological chemistry - 3 Dec 2004
Zhang Min, Hu Peirong, Napoli Joseph L
Abstract excerpt
High affinity, retinoid-specific binding proteins chaperone retinoids to manage their transport and metabolism. Proposing mechanisms of retinoid transfer between these binding proteins and membrane-associated retinoid-metabolizing enzymes requires insight into enzyme topology. We therefore determined the topology of mouse retinol dehydrogenase type 1 (Rdh1) and cis-retinoid androgen dehydrogenase type 1 (Crad1)...
Topics
- Alcohol Oxidoreductases
- Animals
- Arginine
- Blotting, Western
- COS Cells
- Catalytic Domain
- Cytoplasm
- Cytosol
- DNA
- DNA, Complementary
- Endoplasmic Reticulum
- Epitopes
- Gene Deletion
- Glutamine