Article
Functional expression of plant plasma membrane H(+)-ATPase in yeast endoplasmic reticulum.
The Journal of biological chemistry - 15 Jun 1992
Villalba J M, Palmgren M G, Berberián G E, Ferguson C, Serrano R
Abstract excerpt
Recombinant plant plasma membrane H(+)-ATPase has been produced in a yeast expression system comprising a multicopy plasmid and the strong promoter of the yeast PMA1 gene. Western blotting with a specific monoclonal antibody showed that the plant ATPase is one of the major membrane proteins made by the transformed cells, accounting for about 1% of total yeast protein. The plant ATPase synthesized in yeast is...
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